NOT KNOWN FACTS ABOUT 2-FDCK HCL

The lab suggests fixation will bring about the dyes to leak from the cells. I believe the belief is always that fixation may damage cell membranes. I did find a paper which describes fixation applying methanol or acetic acid, immediately after staining with a DCFDA derivative. DCFDA is definitely the reagent that stains ROS during the kit ab113851,